2019-02-14 12:12:252019-02-14 12:09:56
Biological materials
Mutant
MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]
GHB6 ([[gene|ktrC]]::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
GP2264 ([[gene|ktrC]]::''aphA3''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2048 ([[gene|ktrC]]::''cat''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2079 ([[gene|ktrC]]::''tet''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2083 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3'' [[gene|ktrC]]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
BKE14510 ([[gene|ktrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
BKK14510 ([[gene|ktrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]
GHB6 ([[gene|ktrC]]::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jrg Stlke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
GP2264 ([[gene|ktrC]]::''aphA3''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2048 ([[gene|ktrC]]::''cat''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2079 ([[gene|ktrC]]::''tet''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
GP2083 ([[gene|ktrA]]-[[gene|ktrB]]::''aphA3'' [[gene|ktrC]]::''tet''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
BKE14510 ([[gene|ktrC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
BKK14510 ([[gene|ktrC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
Biological materials
Expression vectors
pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab
References
Original publications